Quantifying sponge host and microbial symbiont contribution to dissolved organic matter uptake through cell separation
نویسندگان
چکیده
Sponge-microbe symbioses underpin the ecological success of sponges in many aquatic benthic ecosystems worldwide. These are often described as mutually beneficial, but identifying positive symbiotic interactions and quantifying contribution partners to physiological processes is challenging. For example, our understanding relative sponge cells their microbial symbionts uptake exchange dissolved organic matter (DOM)—a major component diet—is limited. Here, we combined host-symbiont cell separation with pulse-chase isotopic labelling order trace 13 C- 15 Nenriched DOM into encrusting Caribbean Haliclona vansoesti Scopalina ruetzleri , which low abundance (LMA) species. Sponge were responsible for >99% assimilation during experiment both species, while microbes total was negligible (<1%). Nitrogen derived from translocated over time, indicating processing host nitrogenous wastes by endosymbionts. Thus, drive these may aid recycling host-waste products. Our findings highlight ability derive nutrition internalizing compounds environment retaining nutrients via host-microbe interactions.
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ژورنال
عنوان ژورنال: Marine Ecology Progress Series
سال: 2021
ISSN: ['1616-1599', '0171-8630']
DOI: https://doi.org/10.3354/meps13789